《植物生理学报》 2009, 45(9): 843-846

酪氨酸蛋白磷酸酶参与脱氢抗坏血酸的信号途径

范邓鹏¹, 石永春^1,2, 刘卫群^1,2,*

河南农业大学1 国家烟草栽培生理生化重点实验室, 2 生命科学学院, 郑州450002

通信作者：刘卫群；E-mail: liuweiqun2004@126.com；Tel: 0371-63558722

摘　要：

研究酪氨酸蛋白磷酸酶(PTPase)的抑制剂氧化苯胂(PAO)、NaVO3 和 Zn在脱氢抗坏血酸(DHA)调控烟草气孔运动中的作用。结果表明, 0.01 mmol•L-1 PAO、1 mmol•L-1 NaVO 和2 mmol•L-1 Zn2+ 抑制黑暗和DHA诱导的气孔关闭, 而对光诱导的烟草气孔开度的影响不大。据此推测 PTPase 参与 DHA 诱导的气孔关闭信号途径。

关键词：烟草; 酪氨酸蛋白磷酸酶; 脱氢抗坏血酸; 气孔运动; 信号转导

收稿：2009-04-30   修定：2009-08-19

资助：河南省自然科学基金(2007210015)和国家烟草栽培重点实验室科技支撑项目(TCKL06006)。

Involvement of Protein Tyrosine Phosphatase in Signal Pathway of Dehydroascorbate

FAN Deng-Peng¹, SHI Yong-Chun^1,2, LIU Wei-Qun^1,2,*

¹National Key Laboratory of Tobacco Planting and Physiology and Biochemistry, ²College of Life Science, Henan Agricultural Universtiy, Zhengzhou 450002, China

Corresponding author: LIU Wei-Qun; E-mail: liuweiqun2004@126.com; Tel: 0371-63558722

Abstract:

The effects of protein tyrosine phosphatase (PTPase) inhibitor, phenylarsine oxide (PAO), NaVO₃ and Zn²⁺ on the stomatal movement regulated by dehydroascorbate (DHA) were investaged in Nicotiana tabacum.The results indicated that 0.01 mmol·L^-1 PAO, 1 mmol·L^-1 NaVO₃ and 2 mmol·L^-1 Zn²⁺ had no effects on stomatal aperture induced by light, but inhibited the stomatal closure induced by darkness and DHA, suggesting that PTPase might be involved in the signal pathway of stomatal closure induced by DHA.

Key words: Nicotiana tabacum; protein tyrosine phosphatase; dehydroascorbate; stomatal movement; signal transduction